Scientist | Academic Qualification:
Address: Division of Crop Improvement, |
- Transgenic crop development, Molecular Biology of plants and insect-pests, Functional genomics, Transcriptomics, Protein biochemistry and bioinformatics
Designation | Years | Institutions |
---|---|---|
Scientist (undergone Foundation Course on Agricultural Research Service Training) | January, 2014 to March, 2014 | ICAR-National Academy of Agricultural Research Management (NAARM), Hyderabad, INDIA |
Scientist | April, 2014 to present | ICAR-Central Institute for Cotton research, Nagpur, INDIA |
- Co-developer of ICAR-CICR Suraj Bt, variety for insect resistance
- Standardized a medium recipe for healthy root and shoot growth in germinated somatic embryos has been standardized.
- Co-developer of In planta Cotton transformation method: A protocol for genotype independent sonication and vacuum infiltration assisted Agrobacterium tumefaciens mediated in planta method for gene delivery into cotton was standardized for high efficiency
Project title | Funding Agency | Period | Role |
---|---|---|---|
Assessment of nutrient management options in jute seed crop. (Exploratory Project) | In-house / Institute | Completed | Principal Investigator |
Basic Studies on Somatic embryogenesis of Cotton | ICAR-CICR | Project Leader | 2015-2018 |
Development of novel methods for gene delivery into cotton | ICAR-CICR | Project Associate | 2015-2018 |
Gene Discovery for Useful Traits | ICAR-CICR | Project Associate | 2017-2018 |
An efficient regeneration system for transformation studies with CICRcry2Ab1Ac and fiber strength genes in Cotton (G. hirsutum) | ICAR-CICR | Project Associate | 2017-2020 |
Targeted mutagenesis of ghPHYA1 through CRISPR/Cas9 in Cotton | ICAR-CICR | Project Associate | 2017-2018 |
Development of tissue culture technology for cotton fibre initiation. | ICAR-CRP-Natural fibre | Project Associate | 2015-2017 |
Cloning, characterization and inhibitor/s identification for vital proteins involved in chitin biosynthesis pathway of Cotton Pink Bollworm (Pectinophora gossypiella). | ICAR-CICR | Project Leader | 2021-2024 |
Exploration of genomic resources for identification of candidate genes and promoters for cotton improvement. | ICAR-CICR | Project Associate | 2021-2025 |
Phytohormone profiling by targeted metabolomics in cotton. | ICAR-CICR | Project Associate | 2021-2024 |
Research Papers/ Ariticles
- Joy Das, Rakesh Kumar, Vivek Shah, K. P. Raghavendra, and Ashwani Kumar Sharma, (2023). Identification and functional characterisation of N-acetylglucosamine kinase from Helicoverpa armigera divulge its potential role in growth and development via UDP-GlcNAc salvage pathway. International Journal of Biological Macromolecules, 242: 124674.
- Joy Das, Rakesh Kumar, Vivek Shah, Ashwani Kumar Sharma, (2023). Simple cost-effective larval injection method for dsRNA delivery to induce RNAi response in Helicoverpa armigera (Hübner). Journal of Applied Entomology, 147 (4): 289-298.
- Rakesh Kumar, Joy Das, Surabhi Rode, Harry Kaur, Vivek Shah, Pooja Verma and Ashwani Kumar Sharma, (2023). Farnesol dehydrogenase from Helicoverpa armigera (Hübner) as a promising target for pest management: molecular docking, in vitro and insect bioassay studies 3 Biotech, 13(6), 175.
- Md Shahid Alam, Monica Sharma, Rakesh Kumar, Joy Das, Surabhi Rode, Pravindra Kumar, Ramasare Prasad & Ashwani Kumar Sharma, (2023). In silico identification of potential phytochemical inhibitors targeting farnesyl diphosphate synthase of cotton bollworm (Helicoverpa armigera), Journal of Biomolecular Structure and Dynamics, 41:5, 1978-1987.
- Rakesh Kumar., Joy Das, Jai Krishna Mahto, Monica Sharma., Vivek Shah., Pravindra Kumar, Ashwani Kumar Sharma, (2022). Crystal structure and molecular characterization of NADP+-farnesol dehydrogenase from cotton bollworm, Helicoverpa armigera. Insect Biochemistry and Molecular Biology, 147: 103812.
- Joy Das, Rakesh Kumar, Vivek Shah, Ashwani Kumar Sharma, (2022). Functional characterization of chitin synthesis pathway genes, HaAGM and HaUAP, reveal their crucial roles in ecdysis and survival of Helicoverpa armigera (Hübner). Pesticide Biochemistry and Physiology 188, 105273.
- Raghavendra, K.P., Joy Das, Kumar, R., Gawande, S.P., Santosh, H.B., Sheeba, J.A., Kranthi, S., Kranthi, K.R. and Waghmare, V.N., (2021). Genome-wide identification and expression analysis of the plant specific LIM genes in Gossypium arboreum under phytohormone, salt and pathogen stress. Scientific reports, 11(1), pp.1-14.
- Harry Kaur, Rakesh Kumar, Joy Das and Ashwani Kumar Sharma, “Insect metabolic esterases: exploring their potential roles in environmental bioremediation, Abstract published in conference proceeding of International Conference on Biotechnology for Sustainable Agriculture, Environment and Health (BSAEH – 2021) April 4 – 8, 2021 Jaipur, Rajasthan, India
- Harry Kaur, Rakesh Kumar, Joy Das and Ashwani Kumar Sharma “Studies on bioremediation potential of carboxy/choline esterases (CCEs) from Cotton Bollworm (Helicoverpa armigera (Hubner)” Abstract published in conference proceeding of “48th National Seminar on Crystallography” organized by Indian Institute of Technology, Roorkee during November 25 – 27, 2021
- Kumar, R., Joy Das.; Sharma, M., Kumar, P., Sharma, A.K., (2021). Identification of Small-Molecule Potential Inhibitor(s) for Helicoverpa armigera Juvenile Hormone Acid-o-Methyl Transferase (HaJHAMT) through Molecular Docking and MD Simulation Approaches, in Proceedings of the 1st International Electronic Conference on Entomology, 1–15 July 2021, MDPI: Basel, Switzerland. DOI:10.3390/IECE-10570
- Kumar, R., Joy Das, Raghavendra, K. P., & Nandeshwar, S. B., (2020), Identification and expression pattern analysis of two Gossypium hirsutum zinc finger transcription factors during cotton fiber initiation. National Academy Science Letters, 43(2), 115-119.
- Raghavendra, K.P., Kumar, R., Joy Das, Santosh, H.B., More, S.A., CHAWLA, S.G., Kranthi, S. and Kranthi, K.R., (2020). Quantitative real-time PCR based evaluation and validation of reference genes in Gossypium arboreum. The Indian Journal of Agricultural Sciences, 90(1).
- K. Kranthi, K. R., Joy Das, Kumar, R., McCue, M., Dhandapani, Hake, K., Kranthi, S., Blaise, D. & Hughes, (2020). The International Cotton Advisory Committee, Recorder, Volume XXXVIII, No. 2, ISSN 1022-6303
- Joy Das, Rakesh Kumar, K P Raghavendra, CRISPR/CAS9, TALENS and nucleases for gene editing in cotton improvement, (2019). ICAC Recorder: 1007(3): 11-17
- Raghavendra K.P, Joy Das and Rakesh Kumar, (2019). GM event detection and Identification. In: Compendium of Lectures and Training Manual, Short Course on ‘Molecular Approaches for Cotton Improvement’, Eds. Waghmare VN, Raghavendra KP, and Santosh H.B., IASRI, New Delhi. pp: 100-105.
- Mulla, S.R., Joshi, S. and Joy Das, (2018). Isolation and characterization of Photorhabdus luminescens from western ghats of Karnataka. Multilogic In Science, 8(26), pp. 148-151.
- Joy Das, K. P. Raghavendra, H. B. Santosh, M. Sabesh and K. R. Kranthi, Cotton DNA Traceability Technologies. Volume 3 No 3, September 2017, pp. 5-15
- Joy Das, KP Raghavendra, HB Santosh, M Sabesh, KR Kranthi, Cotton DNA traceability technologies, (2017). ICAC Recorder: XXXV (3): 5-15
- Joy Das, Rakesh Kumar, K P Raghavendra, “App”ing for science!!!, Cotton innovate Issue 2, Volume 2, February 2016.
- Mulla, S.R., Joshi, S. and Joy Das, (2017) Isolation, Cloning, and Expression of Insecticidal-Protein-Encoding Gene tcdA from Photorhabdus luminescens in Escherichia coli. International Journal of Current Microbiology and Applied Sciences, 6(9), 1718-1724.
- Raghavendra K.P., Joy Das, Rakesh Kumar, Annie J. Sheeba ,H. B. Santosh and S.P. Gawande “Identification and characterization of lim genes in Gossypium arboreum” Seventh Asian Cotton Research and Development Network (ACRDN) Meeting held on September 15th -17th, 2017. Hotel Le Méridien, Nagpur, Maharashtra
- Rakesh Kumar, Joy Das, S B Nandeshwar, K P Raghavendra and K R Kranthi, “Development of in vitro Nanocomplex for Gene Delivery into Cotton”. Abstracts: “National Conference on Innovations In Agri-Biosciences (NCIABS-2016)” on February 26-27th, 2016, Dr Ambedkar College Nagpur, India. International Journal of life sciences, special issue A6; February 2016.
- Saravanan, M., Joy Das, Misra, R. C., Mohan, P., Waghmare, V.N., and Kranthi, K.R. (2016), Genetic diversity analysis using SSR markers or desi cotton (G. arboreum) landraces collected from Arunachal Pradesh. Cotton Research Journal, 7(2). pp. 91-96
- Joy Das, Rakesh Kumar and Nandeshwar, S. B., (2016). Study of novel genes associated with somatic embryogenesis of Gossypium” National Conference on Innovations in Agri -Biosciences organized by Dr. Ambedkar College Nagpur.
- Joy Das and Rakesh Kumar, (2016). Plant Wnt: deciphering a novel signalling pathway in plants. Current Science, 111(8), p.1319.
- Joy Das, ‘Back-to-nature’ breeding: reliving the wild past’, Cotton innovate Issue 2, Volume 1, January 2015
- Joy Das, Ramesh, K.V., Maithri, U., Mutangana, D. and Suresh, C.K., (2014). Response of aerobic rice to Piriformospora indica. Ind. J Exp. Biol. :52 pp. 237-251
Book Chapters
- Balasubramani, G., K. P. Raghavendra, Joy Das, Rakesh Kumar, H. B. Santosh, J. Amudha, Sandhya Kranthi, and K. R. Kranthi. Critical Evaluation of GM Cotton. 2021, In Cotton Precision Breeding, pp. 351-410. Springer, Cham.
- Raghavendra, K.P, Joy Das, Rakesh Kumar, Santosh H. B., Annie Sheeba, S. P. Gawande, Balasubramani, G. and V. N. Waghmare, Exploration of genomic resources for trait characterization in cotton, Cotton Production Technologies in the Next Decade: Problems & Perspectives, 2020, pp. 9-13, Cotton Research and Development Association, CCS Haryana Agricultural University, Hisar – 125 004, India
- Raghavendra K.P, Joy Das and Rakesh Kumar. 2019. GM event detection and Identification. In: Compendium of Lectures and Training Manual, Short Course on ‘Molecular Approaches for Cotton Improvement’, Eds. Waghmare VN, Raghavendra KP, and Santosh H.B., IASRI, New Delhi. pp: 100-105.
- Participated in the “4th International Conference on Environmental, Agricultural, Chemical and Biological sciences”, organized by Voice of Indian Concern for the Environment (VOICE), Canada, All India Institute of Training and Education (AIITE), New Delhi and Dept. of Biotechnology, Srimad Andavan Arts and Science College, Trichy, Tamil Nadu during May 13-14, 2023 in Online Mode.
- Participated in the “Online hands- on training on LaTex Software” organized by SIAS, Calicut University, Kerala from 21st February to 01st March 2023.
- Participated at the ‘VIIth International Conference in Hybrid Mode on Global Research Initiatives for Sustainable Agriculture & Allied Sciences’ during 21st-23th November, 2022.
- Participated in the International Conference on “Biotechnology Trends on Future Prospects (BTFP)”, held from 13th to 15th September 2022
- Delivered oral presentation at National Symposium on “Paradigm Shift in Cotton Cultivation” organized by CRDA at MPUAT, Udaipur during 08-10 August 2022
- Participated in the International conference on “Innovative and current advances in agriculture and allied sciences” held during June 12th to 14th, 2022 at Himachal Pradesh University, Summer Hill, Shimla, H.P
- 7th Asian Cotton Research and Development Network (ACRDN) meeting-cum-conference organized by The Indian Society of Cotton Improvement (ISCI) Mumbai in colloboration with ICAC, WashingtonDC, USA,CICR, Nagpur and CIRCOT Mumbaiat Hotel Le Meridien, Nagpur, Maharashtra during September 15-17, 2017
- International conference on recent trends in science and technology -sustainable development through scientific approach” organized by Shri Shivaji Education Society’s Science College, congress Nagar, Nagpur during 12, 13 & 14th July 2017.
- National workshop on Application of X-Ray Crystallography for Molecular Structure Determination held from 8th to 9th December, 2016
- 2nd Annual International conference on Emerging trends in Applied Sciences”, held on 22nd and 23rd January, 2016 at Tulsiramji Gaikward Patil College of Engineering and Technology, Nagpur)
- Oral presentation on “Study of novel genes associated with Somatic embryogenesis of Gossypium” at UGC sponsored National Conference on Innovations in Agri-Biosciences NCIABS-2016 held at Dr. Ambedkar College, Deekshabhoomi, Nagpur from 26th to 27th February, 2016.
- National workshop on “Tilling in Crop Plants” held from December 7th to 18th, 2015 at Dept. Of Plant Sciences, University of Hyderabad
- Centre of Advanced Faculty Training Program at ICAR-IASRI, New Delhi 2015
- NAAS Silver Jubilee Symposium held at CICR, Nagpur on December 27, 2014
- AGRO VISION exhibition held at Reshimbag, Nagpur from 04-07, December 2014, explained about the technologies at CICR, Nagpur to the farmers
- Professional attachment training at ICAR-IIHR. Bangalore in 2014
- Foundation Course for Agricultural Research Service (FOCARS), ICAR-NAARM in 2014
Invited Lectures
- Delivered lecture as a resource person for Synergistic Training Program Utilizing the Scientific and Technological Infrastructure (STUTI) organised during 17th to 23rd January 2023 at Sant Gadge Baba Amravati University.
- Innovative club talk on “Plant Genome Engineering and Potential for Cotton improvement “under the ages of Innovative cell ICAR -CICR Nagpur on 13.02.2015
- Delivered an invited talk on “Trends in Molecular Biology and its practical utility in cotton research” on the Brain Storming Session of the Golden Jubilee Celebration on “Fifty Years of Cotton Research – Lessons Learnt and the Way Forward” held from 9th to 10th November, 2016 at ICAR – Central Institute for Cotton Research, Regional Station, Coimbatore
- Delivered a talk on “CRISPR/Cas technology for next-generation cotton: an overview” on the 7th Asian Cotton Research and Development Network Meeting held during 15th-17th Sept. 2017
- Delivered a talk on “CRISPR/Cas technology for next-generation cotton: an overview” on the 7th Asian Cotton Research and Development Network Meeting held during Sept. 2017
Students guided
- Guided a student of M.Sc. Biotechnology namely Miss. Tanvi Subhash Mohod, from Lady Amritbai Daga College, Nagpur under Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur for completion of her dissertation entitled “In silico and molecular studies on chitin synthesis pathway enzyme glucosamine 6-phosphate N-acetyltransferase from Helicoverpa armigera” from January 2023 to May 2023.
- Acted as co-guide for one M.Sc. student for project work entitled “Agrobacterium tumifaciens (strain LBA4404) mediated Gus gene transformation of cotton embryo” in biotechnology from Govt. VYT PG Autonomous college, Durg
- Received “Young Scientist Award” for the work entitled “Unravelling the chitin biosynthesis network in Pink bollworm, a detrimental cotton pest” in the National Symposium on “Paradigm shift in cotton cultivation”, organized by CRDA at MPUAT, Udaipur during 08-10 August 2022
- JNU fellowship for PG studies 2010
- GATE Biotechnology 2012
- DBT JRF 2012
- CSIR 2013
- ICAR-NET 2013
- ARS 2013
- Received Review award for best oral presentation and second-best paper for Oral presentation on “Study of novel genes associated with Somatic embryogenesis of Gossypium” at UGC sponsored National Conference on Innovations in Agri-Biosciences NCIABS-2016 held at Dr. Ambedkar College, Deekshabhoomi, Nagpur from 26th to 27th February, 2016